The variation of anti-SARS-CoV-2 spike trimeric IgG in both cohorts is shown in Fig. coronavirus 2 (SARS-CoV-2) infections, even within healthcare environments [1]. Recent evidence reinforces the concept that vaccinations against SARS-CoV-2 Norisoboldine is effective to limit the burden of healthcare-associated COVID-19 outbreaks [2]. Nonetheless, since it may be supposed that overlapping SARS-CoV-2 infections occurring after administration of vaccine booster doses may contribute to provide a further amplifying stimulus to the humoral response, we investigated the effect of Norisoboldine post-booster SARS-CoV-2 infections on anti-SARS-CoV-2 antibodies elicited by a BNT162b2 vaccine booster in a cohort of baseline SARS-CoV-2-seronegative healthcare workers. 2.?Materials and methods The main characteristics of this retrospective observational SARS-CoV-2 serosurveillance study have been described elsewhere [3]. Briefly, the anti-SARS-CoV-2 spike trimeric IgG were measured with DiaSorin Trimeric spike IgG immunoassay on Liaison XL (DiaSorin, Saluggia, Italy) [4] in healthcare workers undergoing main vaccination with Pfizer/BioNTech BNT162b2 (Pfizer Inc., New York, US; two 30?g doses, with 3-week interval) followed by administration of homologous vaccine booster (30?g single-dose) more than 8?months later. Molecular screening for detecting symptomatic and asymptomatic SARS-CoV-2 infections (Seegene Allplex SARS-CoV-2 Assay; Seegene Inc., South Korea or Altona Diagnostics RealStar SARS-CoV-2 RT-PCR Kit; Altona Diagnostics GmbH, Hamburg, Germany) was conducted at 2C4?weeks intervals by nasopharyngeal swab, throughout the study. Venous blood samples were drawn before either dose of main vaccination, at 1, 3 and 6?months afterwards and, finally, before and 1?month after receiving the homologous vaccine booster dose. Statistical significance (set at em p /em ? ?0.05) of differences in serum anti-SARS-CoV-2 spike trimeric IgG values, expressed as kilo Binding Antibodies Models per litre (kBAU/L), was assessed with Mann-Whitney test, using Analyse-it (Analyse-it Software Ltd, Leeds, UK). Written informed consent for vaccination and participation to the serosurveillance study was obtained from all participants. This observational retrospective study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of Verona and Rovigo Provinces (59COVIDCESC; November 3, 2021). 3.?Results The final study population consisted of 67 baseline seronegative (i.e. pre-vaccination) healthcare workers, 14 with (median age, 42?years; IQR, 31C48?years; 29% of females) or 53 without (median age, 46?years; IQR, 34C54?years, 60% of females) a diagnosis of SARS-CoV-2 contamination within 4?weeks after receiving the homologous booster vaccine dose. The variance of anti-SARS-CoV-2 spike trimeric IgG in both Norisoboldine cohorts is usually shown in Fig. 1 . No significant difference in serum concentration of anti-SARS-CoV-2 spike trimeric IgG levels was observed throughout the study period (all em p /em ? ?0.05) between subjects with or without a diagnosis of post-booster SARS-CoV-2 contamination. Even though median levels of anti-SARS-CoV-2 spike trimeric IgG at 1?month after receiving the booster vaccine dose appeared slightly higher in subjects with post-booster SARS-CoV-2 contamination (11,720?kBAU/L; IQR, 4,543C16,775?kBAU/L) than in those without (8,700?kBAU/L; IQR, 5,463C15,733?kBAU/L), this difference was not statistically significant ( em p /em ?=?0.257). The rate of subjects with protective values (i.e., 264?kBAU/L, corresponding to the 80% limit of COVID-19 vaccine efficacy against symptomatic disease as estimated by Feng et al. [5]) was 100% in both cohorts after booster vaccine dose. Open in a separate windows Fig. 1 Kinetics of serum anti-SARS-CoV-2 spike trimeric RBD IgG antibodies in baseline seronegative recipients of BNT162b2 mRNA-based main vaccination and booster with or without post-booster SARS-CoV-2 contamination. Results are shown as median and interquartile range (IQR). 4.?Conclusion The results of this study provide evidence that this short-term humoral response to COVID-19 vaccine booster may be comparable between healthcare workers with or without a diagnosis of post-booster SARS-CoV-2 contamination. This aspect suggests that the anti-SARS-CoV-2 humoral response, at least with respect to quantitative antibody levels, may not be proportional to COVID-19 vaccine dosage or to the trigger of an overlapping SARS-CoV-2 contamination in vaccinated people. Even Mouse monoclonal to MPS1 though impact of different SARS-CoV-2 lineages on pre-existing immune memory may not.